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- Title
Cytotoxicity of endodontic materials over 6-weeks ex vivo.
- Authors
Brackett, M. G.; Marshall, A.; Lockwood, P. E.; Lewis, J. B.; Messer, R. L. W.; Bouillaguet, S.; Wataha, J. C.
- Abstract
Aim To test the hypothesis that extending the time of a traditional ex vivo cytotoxicity test helps to identify trends in the behaviour of root core materials and sealers, which could ultimately aid in predicting their clinical safety and performance. Methodology Endodontic sealers and core specimens were initially tested in direct contact with L929 fibroblasts for 72 h. Cell response was estimated by measuring cellular succinate dehydrogenase activity relative to Teflon controls. Cytotoxicity (% of more active cells) was reassessed after 1, 3, 4 and 6 weeks, with the specimens stored in a physiologically balanced salt-solution between tests. Results Distinct trends in cytotoxicity among both core materials and sealers were observed over the 6-week test. Four of the six sealers and two of the three core materials showed cell viabilities of <30% of Teflon after 6 weeks (>70% cytotoxicity). Conclusions The current results suggest that some endodontic materials have an elevated biological risk for extended intervals.
- Subjects
ENDODONTICS; DENTISTRY; SUCCINATE dehydrogenase; DEHYDROGENASES; SUCCINIC acid
- Publication
International Endodontic Journal, 2008, Vol 41, Issue 12, p1072
- ISSN
0143-2885
- Publication type
Article
- DOI
10.1111/j.1365-2591.2008.01471.x