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- Title
Mutations of Ki-ras oncogene codon 12 in betel quid chewing-related human oral squamous cell carcinoma in Taiwan.
- Authors
Kuo, M. Y. P.; Jeng, J. H.; Chiang, C. P.; Hahn, L. J.; Kuo, M Y
- Abstract
In Taiwan, there are two million people who have a betel quid chewing habit, and approximately 80% of all oral cancer deaths are associated with this habit. To investigate the incidence and types of Ki-ras codon 12 mutations in oral cancer associated with betel quid chewing, we used a sensitive mutation-specific two-stage polymerase chain reaction (PCR) technique to examine human oral squamous cell carcinomas from formalin-fixed, paraffin-embedded tissues. DNA sequence analysis of PCR products revealed that 6 of 33 (18%) turnout specimens contained Ki-ras codon 12 mutations. Four of the turnouts contained more than one mutation. Three different base changes were detected, resulting from a substitution of wild type glycine (GGT) to either serine (AGT). aspartic acid (GAT) or cysteine (TAT). These results indicate that Ki-ras oncogene activation may play a role in the oncogenesis of betel quid chewing-related human oral squamous cell carcinomas.
- Subjects
TAIWAN; GENETIC mutation; ONCOGENES; BETEL chewing; SQUAMOUS cell carcinoma; ORAL cancer
- Publication
Journal of Oral Pathology & Medicine, 1994, Vol 23, Issue 2, p70
- ISSN
0904-2512
- Publication type
journal article
- DOI
10.1111/j.1600-0714.1994.tb00259.x