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- Title
Effect of Inducers, Incubation Time and Heme Concentration on IC<sub>50</sub> Value Variation in Anti-heme Crystallization Assay.
- Authors
Nguyen Thanh Thuy Nhien; Nguyen Tien Huy; Dinh Thanh Uyen; Deharo, Eric; Pham Thi Le Hoa; Kenji Hirayama; Shigeharu Harada; Kaeko Kamei
- Abstract
Heme detoxification through crystallization into hemozoin has been suggested as a good target for the development of screening assays for new antimalarials. However, comparisons among the data obtained from different experiments are difficult, and the IC50 values (the concentrations of drug that are required to inhibit 50% of hemozoin formation) for the same drug vary widely. We studied the effects of changes in heme concentration (precursor of β-hematin), incubation time and three inducers (SDS, Tween 20 and linoleic acid) on the IC50 of some antimalarials (chloroquine, quinine, amodiaquine, and clotrimazole). The results showed that increasing both inducer concentration and incubation time raised the IC50 of selected antimalarials. Any change in those factors caused the IC50 value to vary. Standardization of assay conditions is, therefore, necessary to increase reproducibility and reduce discrepancies in assay performance. Considering all of the variables, the best choice of inducers is in the order of SDS > Tween 20 > linoleic acid.
- Subjects
HEME; CRYSTALLIZATION; ANTIMALARIALS; LINOLEIC acid; CHLOROQUINE; QUININE; AMODIAQUINE
- Publication
Tropical Medicine & Health, 2011, Vol 39, Issue 4, p119
- ISSN
1348-8945
- Publication type
Article
- DOI
10.2149/tmh.2011-29