We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The effects and mechanism of high-salt medium on eNOS expression in human umbilical vein endothelial cell line.
- Authors
CAO Yu; FANG Yuan; MU Jian-jun; DONG Xiao-liang; WANG Dan; LIU Fu-qiang
- Abstract
Objective To investigate the effects of high-salt medium on the expression of endothelial nitric oxide synthase (eNOS) and related mechan-sms in EA. Hy926 cell line. Methods EA. hy926 cells were treated 48 h with different concentrations of high-salt medium; 137 mmol/L was selected as the target concentration intervention in further experiment. The cells were divided into four groups which received intervention for 48 h: DMEM high-glucose medium group (sodium chloride concentration of 109 mmol/L), high-salt medium (sodium chloride concentration of 137 mmol/L), high-salt medium (sodium chloride concentration of 137 mmol/L) + RNA interference, and hypertonic control group (mannitol concentration of 56 mmol/L). High-performance liquid chromatography was used to assay ADMA concentration in cell supernatant; real-time quantitative PCR and Western blot were used to assay the expressions and actvities of PRMT-1, RhoA, ROCK and eNOS. Results ADMA concentration, PRMT-1 expression, the mRNA expression of RhoA and ROCK actvity increased significantly whereas the protein expression of eNOS significantly reduced in the high-salt group compared with those in the two control groups. High-sat medium + RNA interference restored the above biochemical indicators and upregulated the protein expression of eNOS significantly. Conclusion High-sat medium induces ADMA-mediated RhoA/ Rock-1 pathway activation and suppresses eNOS generation.
- Subjects
NITRIC-oxide synthases; ENDOTHELIAL cells; UMBILICAL veins; CELL lines; SALINITY; REVERSE transcriptase polymerase chain reaction
- Publication
Journal of Xi'an Jiaotong University (Medical Sciences), 2013, Vol 34, Issue 6, p764
- ISSN
1671-8259
- Publication type
Article
- DOI
10.7652/jdyxb201306013