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- Title
Molecular characterization of a Korean porcine epidemic diarrhea virus strain NB1.
- Authors
Hee-Chun Chung; Van Giap Nguyen; Thi My Le Huynh; Hyoung-Joon Moon; Bo-Kyu Kang; Sung-Jae Kim; Hye-Kwon Kim; Seong-Jun Park; Kun-Taek Park; Yong-Ho Park; Bong-Kyun Park
- Abstract
In Korea, for the past 30 years (1987–present), porcine epidemic diarrhea (PED) has been established as an endemic situation in which multiple genogroups of classical G1 and G2b, and the recently introduced pandemic G2a, coexisted. Because of the dynamic nature of the virus, continuous field monitoring for PEDV strains is required. This study is the first to reveal prevalence of PEDV in 9 sampling provinces, with an overall detection rate of 6.70%. Porcine endemic diarrhea virus (PEDV) was present in pigs of all ages, especially in the non-PED vaccinated groups. The highest detection rate was in the finisher group (2.34%), followed by that in the newborn group (1.56%). Secondly, using Sanger sequencing, this study recovered a complete genome (28 005 nucleotides long) of NB1 strain from a farm severely affected by PED. Analyses of nucleotide and deduced amino acid sequences showed that NB1 differed from 18 other Korean PEDV mostly in 4 protein coding genes: ORF1a, ORF1b, S, and N. Two amino acid substitutions (V635E and Y681Q) in the COE and S1D neutralizing epitopes of NB1 resulted in antigenic index alteration of the adjacent sites, one of which contributed to a mutation that escaped neutralizing antibodies.
- Subjects
SOUTH Korea; PORCINE epidemic diarrhea virus; AMINO acid sequence
- Publication
Canadian Journal of Veterinary Research / Revue Canadienne de Recherche Vétérinaire, 2019, Vol 83, Issue 2, p97
- ISSN
0830-9000
- Publication type
Article