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- Title
Absence of Neuroplastin-65 Affects Synaptogenesis in Mouse Inner Hair Cells and Causes Profound Hearing Loss.
- Authors
Carrott, Leanne; Bowl, Michael R.; Aguilar, Carlos; Johnson, Stuart L.; Chessum, Lauren; West, Melissa; Morse, Susan; Dorning, Joanne; Smart, Elizabeth; Hardisty-Hughes, Rachel; Ball, Greg; Parker, Andrew; Barnard, Alun R.; MacLaren, Robert E.; Wells, Sara; Marcotti, Walter; Brown, Steve D. M.
- Abstract
The Neuroplastin gene encodes two synapse-enriched protein isoforms, Np55 and Np65, which are transmembrane glycoproteins that regulate several cellular processes, including the genesis, maintenance, and plasticity of synapses. We found that an absence of Np65 causes early-onset sensorineural hearing loss and prevented the normal synaptogenesis in inner hair cells (IHCs) in the newly identified mouse mutant pitch. In wild-type mice, Np65 is strongly upregulated in the cochlea from around postnatal day 12 (P12), which corresponds to the onset of hearing. Np65 was specifically localized at the presynaptic region of IHCs. We found that the colocalization of presynaptic IHC ribbons and postsynaptic afferent terminals is greatly reduced in pitch mutants. Moreover, IHC exocytosis is also reduced with mutant mice showing lower rates of vesicle release. Np65 appears to have a nonessential role in vision. We propose that Np65, by regulating IHC synaptogenesis, is critical for auditory function in mammals.
- Subjects
NEUROPLASTICITY; SYNAPTOGENESIS; HAIR cells; DEAFNESS prevention; RISK of deafness; EXOCYTOSIS
- Publication
Journal of Neuroscience, 2016, Vol 36, Issue 1, p222
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.1808-15.2016