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- Title
Increased Incidence of Tumor Takes to B16 Melanoma in C57BL/6-J Mice Injected with B16 Products.
- Authors
Kalish, Richard; Brody, Neil I.
- Abstract
The injection of either viable B16 melanoma cells, killed B16 cells, or B16 cell products increased the incidence of melanomas in C57BI/6J mice inoculated with a threshold dose of B16 cells. In all cases the effect was seen whether the facilitating injection was at a site distant from the challenge inoculum or at the same site. Facilitation was seen with B16 cells and products both from <em>in vivo</em> tumors and from tissue culture. However, cells detached from tissue culture flasks with trypsin no longer had facilitating activity. Facilitating activity was found in concentrated culture supernatants centrifuged at 100,000 × <em>g</em> for 1.5 hr and dialyzed. No activity was detected in the less than 10,000 mol wt fraction. Facilitation was not associated with a change in the time of tumor appearance nor with enhanced growth of B16 cells in culture.
- Subjects
TUMORS; MELANOMA; TISSUE culture; TRYPSIN; SERINE proteinases; ORGAN culture
- Publication
Journal of Investigative Dermatology, 1980, Vol 75, Issue 3, p275
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12523542