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- Title
Overexpression of NtSnRK2.2 enhances salt tolerance in Nicotiana tabacum by regulating carbohydrate metabolism and lateral root development.
- Authors
Liu, Minghong; Wang, Jian; Gou, Jianyu; Wang, Xiaoyan; Li, Zhigang; Yang, Xiaoliang; Sun, Shuguang
- Abstract
SnRK2 is a plant-specific protein kinase family implicated in environmental stress tolerance. Individual SnRK2 genes have acquired distinct regulatory properties in response to various environmental stresses. In this study, NtSnRK2.2 , a SnRK2 subclass II member in Nicotiana tabacum L., was cloned and characterised. Sequence alignment analysis showed that SnRK2.2 exhibits widespread sequence differences across Nicotiana species. The tissue expression pattern of NtSnRK2.2 showed a root-predominant expression. To investigate its biological function, NtSnRK2.2 was overexpressed in tobacco, which subsequently resulted in increased soluble sugars and more lateral roots under a normal condition. A salt-stress tolerance assay showed that NtSnRK2.2 -overexpressing plants exhibited enhanced salt tolerance, which was further confirmed based on its better root architecture and increase in soluble sugars, thereby implying that NtSnRK2.2 is a multifunctional regulatory factor in plants. Together, our results indicated the possible role played by NtSnRK2.2 in maintaining metabolic homeostasis via the regulation of carbohydrate metabolism in response to environmental stress. Individual SnRK2 genes might function differently in environmental stress tolerance. NtSnRK2.2 in N. tabacum had a root-predominant expression pattern. Function analysis showed that NtSnRK2.2 was involved in carbohydrate metabolism and lateral root development. The overexpression of NtSnRK2.2 resulted in enhanced tolerance to salt stress. Therefore, NtSnRK2.2 is a multifunctional regulatory factor in plants and has the potential to be used in transgenic breeding to improve salt stress tolerance in crops.
- Subjects
CARBOHYDRATE metabolism; ROOT development; TOBACCO; DROUGHT tolerance; METABOLIC regulation
- Publication
Functional Plant Biology, 2020, Vol 47, Issue 6, p537
- ISSN
1445-4408
- Publication type
Article
- DOI
10.1071/FP19299