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- Title
Development of an optimized system for electroporation of Listeria species.
- Authors
Alexander, Janet E.; Andrew, P. W.; Jones, Dorothy; Roberts, I. S.
- Abstract
Electroporation was used to facilitate transformation of Listeria species with plasmid DNA. Optimal conditions for transformation of L. monocytogenes were a field strength of 8·5 kV/cm, 200 Ohms resistance, 25 μF capacitor with a time constant of 5 ms. With these conditions, 3·9 × 106 transformants/pg DNA were obtained. Under the same conditions, L. innocua and L. iuanouii exhibited a frequency of transformation similar to that of L. monocytogenes but a somewhat lower level was obtained with L. seeligeri.
- Publication
Letters in Applied Microbiology, 1990, Vol 10, Issue 4, p179
- ISSN
0266-8254
- Publication type
Article
- DOI
10.1111/j.1472-765X.1990.tb00109.x