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- Title
Measurement of Funny Current (I<sub>f</sub>) Channel mRNA in Human Atrial Tissue: Correlation with Left Atrial Filling Pressure and Atrial Fibrillation.
- Authors
Ling-Ping Lai; Ming-Jai Su; Jiunn-Lee Lin; Chang-Her Tsai; Fang-Yue Lin; Yih-Sharng Chen; Juey-Jen Hwang; Stephen Huang, Shoei K.; Yung-Zu Tseng; Wen-Pen Lien
- Abstract
Introduction: The funny current (If) contributes to phase IV spontaneous depolarization in cardiac pacemaker tissue. Enhanced If activity in myocardial tissue may lead to increased automaticity and therefore tachyarrhythmia. We measured the amount of If activity in the messenger ribonucleic acid (mRNA) in human atrial tissue and correlated the mRNA amount to left atrial filling pressure and atrial fibrillation (AF). Methods and Results: A total of 34 patients undergoing open heart surgery were included (15 men and 19 women, aged 55 ± 10 years). Atrial tissue was obtained from the right atrial free wall, the right atrial appendage, the left atrial free wall, and the left atrial appendage, respectively. The mRNA amount of the If channel was measured by reverse transcription polymerase chain reaction and was normalized to the mRNA levels of glyceraldehyde 3-phosphate dehydrogenase. We found that the If channel mRNA was present at ail the atrial sampling sites. A higher left atrial filling pressure, an indicator of congestive bean failure, was associated with a higher If mRNA level (r² = 0.446, P < 0.01 by linear regression). We also found that the mRNA amount was significantly higher in patients with AF than in patients without AF (1.68 ± 0.49 vs 1.27 ± 0.43; P < 0.05). Age, sex, right atrial filling pressure, left atrial dimension, and left ventricular ejection fraction had no significant effect on the mRNA level. conclusion: The mRNA of the If channel is present in the free-wall area and appendage area from both atria. Increased left atrial filling pressure and clinical AF are associated with increased If mRNA level.
- Subjects
ATRIAL fibrillation; CARDIAC pacemakers; MESSENGER RNA; POLYMERASE chain reaction; HEART failure; TACHYCARDIA
- Publication
Journal of Cardiovascular Electrophysiology, 1999, Vol 10, Issue 7, p947
- ISSN
1045-3873
- Publication type
Article
- DOI
10.1111/j.1540-8167.1999.tb01265.x