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- Title
Genome Survey and Transcriptome Analysis on Mycelia and Primordia of Agaricus blazei.
- Authors
Lu, Yuan-Ping; Liao, Jian-Hua; Guo, Zhong-Jie; Cai, Zhi-Xin; Chen, Mei-Yuan
- Abstract
Agaricus blazei, a type of edible straw-rotting mushroom with somewhat sweet taste and fragrance of almonds, has attracted considerable scientific and practical attention. High-throughput Illumina PE150 and PacBio RSII platform were employed to generate a genomic sequence. De novo assembly generated 36 contigs with 38,686,133 bp in size, containing 10,119 putative predicted genes. Additionally, we also studied transcriptional regulation of the mycelia and the primordia for exploration of genes involved in fruiting body formation. Expression profiling analysis revealed that 2,164 genes were upregulated in mycelia and 1,557 in primordia. Functional enrichment showed that differentially expressed genes associated with response to stress, ribosome biogenesis, arginine biosynthesis, and steroid biosynthesis pathway were more active in fruiting body. The genome and transcriptome analysis of A. blazei provide valuable sequence resources and contribute to our understanding of genes related to the biosynthesis pathway of polysaccharide and benzaldehyde, as well as the fruiting body formation.
- Subjects
ALDEHYDE analysis; STEROIDS analysis; ARGININE; BIOCHEMISTRY; GENOMES; PHENOMENOLOGY; EDIBLE mushrooms; POLYSACCHARIDES; GENE expression profiling; SEQUENCE analysis
- Publication
BioMed Research International, 2020, p1
- ISSN
2314-6133
- Publication type
Article
- DOI
10.1155/2020/1824183