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- Title
An Automated Real-Time PCR Assay versus Next-Generation Sequencing in the Detection of BRAF V600 Mutations in Melanoma Tissue Samples.
- Authors
Lenders, Daniela; Bonzheim, Irina; Hahn, Matthias; Gassenmaier, Maximilian; Aebischer, Valentin; Forschner, Andrea; Lenders, Max Matthias; Flatz, Lukas; Forchhammer, Stephan
- Abstract
Background: Next-generation sequencing (NGS) is the most commonly used method for determining BRAF mutational status in patients with advanced melanoma. Automated PCR-based methods, such as the IdyllaTM system, are increasingly used for mutation diagnostics, but it is unclear what impact the choice of diagnostic method has on the management of melanoma. Objectives: To compare the concordance rate of BRAF V600 mutational analysis using IdyllaTM and NGS and to analyze the technical and clinical turnaround time. The clinical relevance is compared by analyzing the impact on the treatment decision. Methods: In this monocentric prospective cohort study, the BRAF mutation status of 51 patients was determined using both methods in parallel. Results: BRAF V600 mutation was detected in 23/51 cases (45%). IdyllaTM showed a 100% concordant result with a faster turnaround time (0.2 days) compared to NGS (12.2 days). In general, less tumor material was required for IdyllaTM than for NGS. Most patients received immunotherapy as a first-line therapy regardless of the BRAF V600 status. Conclusions: IdyllaTM testing proved to be a reliable and rapid alternative to NGS in the determination of BRAF V600 mutation. Although BRAF. status was available earlier, this had no influence on the treatment decision in most cases.
- Subjects
NUCLEOTIDE sequencing; TURNAROUND time; BRAF genes; COHORT analysis; LONGITUDINAL method
- Publication
Diagnostics (2075-4418), 2024, Vol 14, Issue 15, p1644
- ISSN
2075-4418
- Publication type
Article
- DOI
10.3390/diagnostics14151644