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- Title
Differential clinical impacts of tumour budding evaluated by the use of immunohistochemical and haematoxylin and eosin staining in stage II colorectal cancer.
- Authors
Yamadera, Masato; Shinto, Eiji; Kajiwara, Yoshiki; Mochizuki, Satsuki; Okamoto, Koichi; Hase, Kazuo; Ueno, Hideki; Shimazaki, Hideyuki
- Abstract
Aims: The aim of this study was to clarify the quantitative and qualitative differences in tumour budding identification between haematoxylin and eosin (H&E) staining and immunohistochemical (IHC) staining for cytokeratin, and to estimate the respective clinical impacts in stage II colorectal cancer. Methods and results: We retrospectively examined 314 surgically resected cases of stage II colorectal cancer, and assessed tumour budding on serial section slides with H&E staining and IHC staining for cytokeratin. Tumour budding counts based on cytokeratin‐stained slides were strongly correlated with those based on H&E‐stained slides, and had higher detection and reproducibility. On the basis of receiver operating characteristic analyses, the optimal cut‐off values of budding counts for relapse‐free survival (RFS) were 7 and 16 in a ×200 microscopic field with H&E and IHC staining, respectively. With these cut‐off values, tumour budding based on H&E staining had a significant correlation with RFS (80.3% and 93.2% of 5‐year RFS in the high‐budding group and the low‐budding group, respectively), and similar results were observed for IHC staining (79.9% and 91.7%, respectively). The Akaike Information Criterion value for RFS with H&E staining was favourable as compared with that with IHC staining. Conclusions: Tumour budding counts based on cytokeratin‐stained slides showed higher detection and better reproducibility, but did not have as satisfactory clinical impacts as those based on H&E staining.
- Subjects
COLON cancer; KERATIN; PROGNOSIS; CANCER relapse; TUMOR budding
- Publication
Histopathology, 2019, Vol 74, Issue 7, p1005
- ISSN
0309-0167
- Publication type
Article
- DOI
10.1111/his.13830