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- Title
The production and characterization of monoclonal antibodies against Photobacterium damselae ssp. piscicida and initial observations using immunohistochemistry.
- Authors
Jung, T S; Thompson, K D; Morris, D J; Adams, A; Sneddon, K
- Abstract
Five monoclonal antibodies (MAbs: F2B1, 1E4, 13B10, 4D4 and F3G12) were produced against lysed Photobacterium damselae ssp. piscicida (Ph. d. ssp. piscicida). The MAbs recognized three antigens of differing molecular weight on the Western blot of Ph. d. ssp. piscicida. They also cross-reacted with five different species of Vibrio. An enzyme linked immunosorbent assay (ELISA) with MAbs, F3G12 and 4D4 demonstrated differences between Ph. d. ssp. piscicida and three Ph. d. ssp. damselae type strains, indicating differences in the surface antigenicity between these two groups of bacteria. Antigen retrieval in conjunction with immunohistochemistry (IHC) using MAb 13B10, revealed colonies of bacteria in the kidney, spleen and liver of sea bass, Dicentrarchus labrax, infected with pasteurellosis. A number of positive colonies were observed around the mucosal layers of the intestinal tissue, especially within the lamina propria. In addition, a number of bacterial colonies were associated with red blood cells and blood vessels of the organs examined.
- Subjects
PHOTOBACTERIUM; MONOCLONAL antibodies; ANTIGEN-antibody reactions
- Publication
Journal of Fish Diseases, 2001, Vol 24, Issue 2, p67
- ISSN
0140-7775
- Publication type
Article
- DOI
10.1046/j.1365-2761.2001.00260.x