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- Title
Effects of antioxidative agents on apoptosis induced by ischaemia-reperfusion in rat intestinal mucosa.
- Authors
Kojima, M.; Iwakiri, R.; Wu, B.; Fujise, T.; Watanabe, K.; Lin, T.; Amemori, S.; Sakata, H.; Shimoda, R.; Oguzu, T.; Ootani, A.; Tsunada, S.; Fujimoto, K.
- Abstract
Summary Background & aim : We have previously demonstrated that ischaemia-reperfusion induces apoptosis in the intestinal mucosa. To evaluate that reactive oxygen species enhanced intestinal apoptosis after ischaemia-reperfusion, we examined whether antioxidants reduced apoptosis. Methods : Rats were infused through a duodenal tube with antioxidative agents, glutathione, rebamipide and dymethylsulfoxide during 2 h before an ischaemic insult. The superior mesenteric artery was occluded for 60 min, followed by 60 min reperfusion. Apoptosis was evaluated by percentage fragmented DNA (fragmented DNA/total DNA) and immunochemical staining. Results : Increase in apoptosis in the intestinal mucosa after ischaemia-reperfusion was attenuated by intraduodenal infusion of antioxidative agents, but was not completely abolished. Conclusion : Scavenging effects of the antioxidative agents attenuated increases in intestinal apoptosis, indicating that oxidative stress after ischaemia-reperfusion plays an important role in induction of apoptosis in the intestinal mucosa.
- Subjects
APOPTOSIS; INTESTINAL mucosa; REPERFUSION injury
- Publication
Alimentary Pharmacology & Therapeutics, 2003, Vol 18, p139
- ISSN
0269-2813
- Publication type
Article
- DOI
10.1046/j.1365-2036.18.s1.16.x