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- Title
Generation of Expressed Sequence Tags and development of microsatellite markers for Castanopsis sieboldii var. sieboldii (Fagaceae).
- Authors
Ueno, Saneyoshi; Aoki, Kyoko; Tsumura, Yoshihiko
- Abstract
• Castanopsis sieboldii var. sieboldii is an evergreen broadleaved canopy tree that grows on Honshu, Shikoku and Kyushu Islands in Japan. A closely-related, hybridizing, congener species, C. cuspidata var. cuspidata, has different leaf epidermis structure and different seed nuts morphology compared to C. sieboldii var. sieboldii. Furthermore, the habitats in which the two species grow often indicate different water requirements. • Analysis of genetic diversity, using transcribed sequences, will provide a sound basis for the management of this species, allowing successful reforestation, incorporating phylogeographic conservation. In order to obtain transcripts sequences for the species and to develop transcript-based markers, we constructed and analyzed a cDNA library, surveyed microsatellite sequences within it and designed PCR primers for the microsatellites. • The cDNA library was constructed using tissue from the inner bark of C. sieboldii var. sieboldii; 3 354 Expressed Sequence Tags (ESTs) were identified. We constructed 2 417 putative unigenes and assigned putative functions to 1 856 of them. Three hundred and fourteen microsatellites were found within the putative unigenes and 16 EST-SSR (Simple Sequence Repeat) markers were developed. The EST-SSR markers developpe in this study should facilitate future analysis of the genetic diversity of this and related species.
- Subjects
JAPAN; MICROSATELLITE repeats; FAGACEAE; BEECH; FORESTS &; forestry; GRASSLAND restoration
- Publication
Annals of Forest Science (EDP Sciences), 2009, Vol 66, Issue 5, p1
- ISSN
1286-4560
- Publication type
Article
- DOI
10.1051/forest/2009037