We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Anthocyanins in cowpea [ Vigna unguiculata (L.) Walp. ssp. unguiculata].
- Authors
Ha, Tae; Lee, Myoung-Hee; Jeong, Yu; Lee, Jin; Han, Sang-Ik; Park, Chang-Hwan; Pae, Suk-Bok; Hwang, Chung-Dong; Baek, In-Youl; Park, Keum-Yong
- Abstract
The aim of this study was to isolate and identify the anthocyanins in the black seed coated cowpea [ Vigna unguiculata (L.) Walp. ssp. unguiculata] using reverse phase C-18 open column chromatography and high performance liquid chromatography (HPLC) with diode array detection and electro spray ionization/mass spectrometry (DAD-ESI/MS) analysis, respectively. Anthocyanins were extracted from the coat of black cowpea with 1% trifluoroacetic acid (TFA) in methanol, isolated by RP-C-18 column chromatography, and their structures elucidated by 1D and 2D nuclear magnetic resonance (NMR) spectroscopy. The isolated anthocyanins were characterized as delphinidin-3- O-glucoside ( 2) and cyanidin-3- O-glucoside ( 4). Furthermore, 5 minor anthocyanins were detected and identified as delphinidin-3- O-galactoside ( 1), cyanidin-3- O-galactoside ( 3), petunidin-3- O-glucoside ( 5), peonidin-3- O-glucoside ( 7), and malvidin-3- O-glucoside ( 8) based on the fragmentation patterns of HPLC-DADESI/MS analysis.
- Publication
Food Science & Biotechnology, 2010, Vol 19, Issue 3, p821
- ISSN
1226-7708
- Publication type
Article
- DOI
10.1007/s10068-010-0115-x