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- Title
Sensitive, Simple, and Quick Method for Simultaneous Quantification of Clozapine and Amlodipine using Reverse Phase HPLC.
- Authors
Jia Le Lim; Rajagopal, Mogana; Akowuah, Gabriel Akyirem; Keshavarzi, Fazlollah
- Abstract
Hypertension is one of the most prevalent non-communicable diseases in the Malaysian population. In addition, it is associated with various mood and psychiatric disorders such as schizophrenia. Recent studies have shown that hypertension leads to worse clinical outcomes in psychiatric patients. Diverse psychosocial factors caused by schizophrenia also impact hypertension and its clinical care. Clozapine and amlodipine are the hallmark drugs for the treatment of schizophrenia and hypertension, respectively. To date, there is no analytical assay that can simultaneously determine the concentrations of these two drugs in human matrices. An Aquasil C18 column was used in the analysis, with the mobile phase consisting of acetonitrile:water (40:60 v/v) adjusted to pH 4.5 with phosphate buffer. Detection with UV spectrometer was done at 240nm. The method was validated according to the International Council for Harmonization (ICH) guidelines. The calibration curves of clozapine and amlodipine were linear in the range of 100-600 ng/mL and 2-12 ng/mL, respectively (r2>0.99). Clozapine limit of detection (LOD) and limit of quantification (LOQ) were 129.5 ng/mL and 392.4 ng/mL, respectively. Meanwhile, amlodipine achieved LOD of 0.25 ng/mL, and LOQ of 0.75 ng/mL. Overall, the study describes quick, precise and sensitive HPLC-UV method for the simultaneous determination of clozapine and amlodipine in human plasma.
- Subjects
AMLODIPINE; CLOZAPINE; MALAYSIANS; HIGH performance liquid chromatography; PEOPLE with mental illness; OLANZAPINE; CALCIUM antagonists; ARIPIPRAZOLE
- Publication
Current Trends in Biotechnology & Pharmacy, 2023, Vol 17, p42
- ISSN
0973-8916
- Publication type
Article