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- Title
Post-transcriptional control of a glucoamylase gene fromTrichoderma harzianumunder stress conditions.
- Authors
Dana, María de las Mercedes; Pintor-Toro, José A.
- Abstract
We have examined the regulation ofTrichoderma harzianumglucoamylase gene (Gla66) in response to different growth conditions. Transcription of theGla66gene is initiated from two different sites, yielding two transcripts of 2.1 kb and 2.6 kb respectively. The 2.1 kb mRNA (ST) encodes for an extracellular glucoamylase of 66 kDa. This protein shows the domains conserved in other fungal glucoamylases: a signal peptide responsible for protein secretion and a catalytic domain, both joined by a linker region. The longest transcript (LT) is untranslated, it contains an unusually extended 5′-untranslated region and is transcribed under stress and growth limiting conditions. The translational control ofLTcould be defined by the presence of four upstream open reading frames (uORFs) in its 5′-leader sequence. The analysis of these uORFs in a yeast heterologous system shows that two of these uORFs prevent theGla66translation under unfavourable growth conditions, when theLTtranscript is accumulated.
- Subjects
TRICHODERMA; AMYLASES; GLYCOSIDASES; MICROBIAL enzymes; BACTERIAL proteins; BACTERIAL genetics; MICROBIAL genetics
- Publication
Molecular Microbiology, 2005, Vol 57, Issue 1, p250
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/j.1365-2958.2005.04682.x