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- Title
Bone morphogenetic protein 7 polarizes THP-1 cells into M2 macrophages.
- Authors
Rocher, Crystal; Singla, Reetu; Singal, Pawan K.; Parthasarathy, Sampath; Singla, Dinender K.
- Abstract
It was hypothesized that monocyte treatment with bone morphogenetic protein 7 (BMP7) would significantly enhance monocyte polarization into M2 macrophages as well as increasing the levels of anti-inflammatory cytokines. In a cell culture system using monocytes (human acute monocytic leukemia cell line THP-1), we studied the effects of BMP7 on monocytes polarizing into M2 macrophages. The data demonstrate that THP-1 cells contain a BMP type II receptor (BMPR2), and that its activation is significantly (p < 0.05) increased following treatment with BMP7. Furthermore, there was an increase of M2 macrophages, BMPR2, and anti-inflammatory cytokines interleukin (IL)-10 and IL-1ra compared with the respective controls. Moreover, treatment with BMP7 caused a significant (p < 0.05) decrease in the levels of proinflammatory cytokines IL-6, tumour necrosis factor (TNF-α), and monocyte chemotactic protein-1 (MCP-1), compared with the controls. In conclusion, we suggest for the first time that BMP7 has a unique potential to polarize monocytes into M2 macrophages, required for tissue repair, which will have significant applications for the treatment of atherosclerosis.
- Subjects
HEALTH outcome assessment; MACROPHAGES; CYTOKINES; BONE morphogenetic proteins; MONOCYTES; ATHEROSCLEROSIS treatment
- Publication
Canadian Journal of Physiology & Pharmacology, 2012, Vol 90, Issue 7, p947
- ISSN
0008-4212
- Publication type
Article
- DOI
10.1139/Y2012-102