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- Title
Enhanced collection of phenotypic and engrafting human cord blood hematopoietic stem cells at 4°C.
- Authors
Broxmeyer, Hal E.; Cooper, Scott; Capitano, Maegan L.
- Abstract
The number of hematopoietic stem cells (HSCs) collected in cord blood (CB) at the birth of a baby is a limiting factor for efficacious use of CB in hematopoietic cell transplantation (HCT). We now demonstrate that collecting and processing of human CB at 4°C within minutes of the baby's birth results in significantly enhanced numbers of rigorously defined phenotypic HSC and self‐renewing NSG immune‐deficient mouse engrafting and SCID‐repopulating cells. This was associated with decreased numbers of hematopoietic progenitor cells (HPC), as noted previously for hypoxia collected/processed cells blocking ambient air induced differentiation of HSC to HPC. We have thus defined a simple, cost‐effective, means to collect increased numbers of CB HSC, of potential use for clinical CB HCT.
- Subjects
CORD blood; HEMATOPOIETIC stem cells; CELL transplantation; CELL differentiation; HYPOXEMIA
- Publication
Stem Cells, 2020, Vol 38, Issue 10, p1326
- ISSN
1066-5099
- Publication type
Article
- DOI
10.1002/stem.3243