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- Title
IFATS Collection: Selenium Induces Improvement of Stem Cell Behaviors in Human Adipose-Tissue Stromal Cells via SAPK/JNK and Stemness Acting Signals.
- Authors
Jeong Hwan Kim; Mi Ran Lee; Jee Hun Kim; Min Ki Jee; Soo Kyung Kang
- Abstract
In the present study, the potential of selenium to enhance stem cell behavior through improvement of human adipose tissuederived stromal cells (ATSCs) and the associated molecular mechanism was evaluated. Selenium-induced improvement in stem cell behavior of human ATSCs caused expression of several genes, indicating downregulated mature cell marker proteins coupled with increased cell growth and telomerase activities after the overexpression of Rex1, Nanog, OCT4, SOX2, KLF4, and c-Myc. Also, selenium-treated ATSCs significantly downregulated p53 and p21 tumor suppressor gene products. Selenium induced active growth and growth enhanced by the activation of signal proteins in ATSCs via the inhibition of reactive oxygen species-mediated phospho-stressactivated protein kinase/c-Jun N-terminal protein kinase activation. The selenium-induced activation of extracellular regulated kinases 1/2 and Akt in ATSCs resulted in a subsequent induction of the expression of stemness transcription factors, particularly Rex1, Nanog, and Oct4, along with definitive demethylation on regulatory regions of Rex-1, Nanog, and Oct4. The results of our small interfering RNA knockdown experiment showed that Rex1 plays a major role in the proliferation of selenium-induced ATSCs. Selenium-treated ATSCs also exhibited more profound differentiation into mesodermal and neural lineages. We performed a direct comparison of gene expression profiles in control ATSCs and selenium-treated ATSCs and delineated specific members of important growth factor, signaling, cell adhesion, and transcription factor families. The observations of improved life span and multipotency of selenium-treated ATSCs clearly indicate that seleniumtreated ATSCs represent an extraordinarily useful candidate cell source for tissue regeneration.
- Subjects
CONNECTIVE tissues; GRANULOCYTE-macrophage colony-stimulating factor; COLONY-stimulating factors (Physiology); CYTOKINES; GROWTH factors; NERVE tissue proteins
- Publication
Stem Cells, 2008, Vol 26, Issue 10, p2724
- ISSN
1066-5099
- Publication type
Article
- DOI
10.1634/stemcells.2008-0184