We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Involvement of β1 and β2 Integrin Stimulation in RNA Synthesis in an Eosinophilic Cell Line (EoL-1).
- Authors
Tsuda, Akira; Saito, Norihiro; Kayaba, Hiroyuki; Kamada, Yumiko; Oyamada, Hajime; Chihara, Junichi
- Abstract
In allergic inflammatory disease, especially in bronchial asthma, eosinophils play important roles as essential inflammatory cells. In the accumulation of eosinophils in airway inflammation, eosinophils receive very diverse stimuli. In this study, we investigated the influences of the signal between β[sub 2] integrin/intercellular adhesion molecule-1 (ICAM-1) or β[sub 1] integrin/fibronectin (FN) and RNA synthesis on proteins in eosinophilic cell line-1 (EoL-1), using recombinant soluble ICAM-1 (r-sICAM-1) as a global response of eosinophils. [sup 3] H-thymidine incorporation and [sup 3] H-uridine incorporation were used as indices for DNA synthesis and RNA synthesis, respectively. In a comparison of the RNA/DNA ratio with various durations of stimulation of 1 μg/ml of r-sICAM-1 and 100 μg/ml of FN, a time-dependent increase was observed, but the increase induced by FN rose slower than that induced by r-sICAM-1. From this result, a β[sub 2] integrin/ICAM-1 signal induced an increase in the RNA/DNA ratio in EoL-1, implying that the signal promotes RNA synthesis, which suggests that various types of protein synthesis, such as the synthesis of various cytokines, are induced by the β[sub 2] integrin/ICAM-1 signal. Similar results were obtained with a β[sub 1] integrin signal using FN, but there was a difference in the time course between β[sub 2] integrin/ICAM-1 and β[sub 1] integrin/FN signals. This experimental method may be useful for understanding these manifestations as a global response of eosinophils.
- Subjects
ASTHMA; BRONCHIAL diseases; FIBRONECTINS; CELL lines; CELL adhesion molecules; EOSINOPHILS; INTEGRINS
- Publication
International Archives of Allergy & Immunology, 1998, Vol 117, p44
- ISSN
1018-2438
- Publication type
Article
- DOI
10.1159/000053570