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- Title
Identification of the Disease-Related T Cell Epitope of Ovalbumin and Epitope-Targeted T Cell Inactivation in Egg Allergy.
- Authors
Shimojo, Naoki; Katsuki, Toshiyuki; Coligan, John E.; Nishimura, Yasuharu; Sasazuki, Takehiko; Tsunoo, Hajime; Sakamaki, Tateo; Kohno, Yoichi; Niimi, Hiroo
- Abstract
An ovalbumin (OVA)-specific T cell line (TCL) was established from a patient with hen egg allergy. The TCL was CD4+, expressed αβ T cell receptor, and recognized OVA presented by HLA-DR10. Based on the response of the TCL to synthetic OVA peptides, it was found that the TCL recognized OVA 323-339, which is a major T cell epitope presented by murine I-Ad. The TCL secreted high levels of IL-5, but undetectable amounts of IL-2, interferon-γ, and IL-4 when stimulated with OVA or the OVA 323-339 peptide. Since IL-5 is an important growth and chemotactic factor for eosinophils, it is possible that these OVA 323-339-specific T cells can contribute to human egg allergy. To our knowledge, this is the first demonstration of food allergen-specific TCL establishment and identification of a T cell epitope possibly related to the allergic reaction to food antigens. An analog peptide of the OVA 323-339 which is known to strongly bind to I-Ad partially inhibited the response of the TCL to OVA 323-339 presented by HLA-DR10, raising the possibility of peptide-based immunotherapy of food allergy. Copyright © 1994 S. Karger AG, Basel
- Publication
International Archives of Allergy & Immunology, 1994, Vol 105, Issue 2, p155
- ISSN
1018-2438
- Publication type
Article
- DOI
10.1159/000236818