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- Title
Growth rate of a non-fermentative Escherichia coli strain is influenced by NAD<sup>+</sup> regeneration.
- Authors
Vázquez-Limón, Consuelo; Vega-Badillo, Joel; Martínez, Alfredo; Espinosa-Molina, Gabriela; Gosset, Guillermo; Soberón, Xavier; López-Munguía, Agustín; Osuna, Joel
- Abstract
By complementing a non-fermentative Escherichia coli ( ldhA − pflB −) strain with the recombinant Zymomonas mobilis ethanol pathway ( pdc, adhB), we evaluated the effect of different levels of enzymatic activity on growth rate demonstrating that there is a direct relationship between anaerobic growth rate and the total specific activity of pyruvate decarboxylase, which is the limiting enzyme of this specific fermentative NAD+ regenerating pathway. This relationship was proved to be useful to establish a selection strategy based on growth rate for the analysis of lctE libraries, which encode lactate dehydrogenase from Bacillus subtilis.
- Subjects
ESCHERICHIA coli; RECOMBINANT microorganisms; ZYMOMONAS mobilis; BACTERIAL growth; ANAEROBIC bacteria; GRAM-negative bacteria; DEHYDROGENASES; REGENERATION (Biology); ENZYMES
- Publication
Biotechnology Letters, 2007, Vol 29, Issue 12, p1857
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-007-9481-8