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- Title
Transgenic expression in tobacco of a poly-proctolin construct leading to production of the bioactive peptide.
- Authors
Rao, Rosa; Breuer, Michael; Tortiglione, Claudia; Malva, Carla; Baggerman, Gilon; Corrado, Giandomenico; De Loof, Arnold; Pennacchio, Francesco
- Abstract
Short peptides can be expressed in plants using synthetic genes encoding multiple copies of the peptide spaced by dibasic endoproteolytic cleavage sites. A synthetic gene encoding an array of repeated copies of proctolin, a very well characterized insect myotropic peptide, spaced by Arg residues, was synthesized and expressed in tobacco plants. The successful production of bioactive proctolin from the precursor in transgenic plants was demonstrated by immunoblot, HPLC, mass spectrometry and a bioassay based on the contraction of isolated cockroach hindgut. These results suggest thatin planta, as in animals and yeasts, endopeptidases of the serine proteases family may be involved in precursor processing.
- Subjects
TOBACCO; PLANT genetic engineering; TRANSGENIC plants; PEPTIDES; PROTEINS; PLANT proteins
- Publication
Biotechnology Letters, 2004, Vol 26, Issue 18, p1413
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/B:BILE.0000045644.52747.6a