We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Effect of diindolylmethane on Ca movement and viability in HA59T human hepatoma cells.
- Authors
Cheng, Jin-Shiung; Shu, Su-Shung; Kuo, Chun-Chi; Chou, Chiang-Ting; Tsai, Wei-Lun; Fang, Yi-Chien; Kuo, Li-Ni; Yeh, Jeng-Hsien; Chen, Wei-Chuan; Chien, Jau-Min; Lu, Ti; Pan, Chih-Chuan; Cheng, He-Hsiung; Chai, Kuo-Liang; Jan, Chung-Ren
- Abstract
The effect of diindolylmethane, a natural compound derived from indole-3-carbinol in cruciferous vegetables, on cytosolic Ca concentrations ([Ca]) and viability in HA59T human hepatoma cells is unclear. This study explored whether diindolylmethane changed [Ca] in HA59T cells. The Ca-sensitive fluorescent dye fura-2 was applied to measure [Ca]. Diindolylmethane at concentrations of 1-50 μM evoked a [Ca] rise in a concentration-dependent manner. The signal was reduced by removing Ca. Diindolylmethane-induced Ca influx was not inhibited by nifedipine, econazole, SK&F96365, and protein kinase C modulators but was inhibited by aristolochic acid. In Ca-free medium, treatment with the endoplasmic reticulum Ca pump inhibitors thapsigargin or 2,5-di-tert-butylhydroquinone (BHQ) inhibited or abolished diindolylmethane-induced [Ca] rise. Incubation with diindolylmethane inhibited thapsigargin or BHQ-induced [Ca] rise. Inhibition of phospholipase C with U73122 reduced diindolylmethane-induced [Ca] rise. At concentrations of 10-75 μM, diindolylmethane killed cells in a concentration-dependent manner. The cytotoxic effect of diindolylmethane was not reversed by chelating cytosolic Ca with 1,2-bis(2-aminophenoxy)ethane- N, N, N′, N′-tetraacetic acid. Propidium iodide staining data suggest that diindolylmethane (25-50 μM) induced apoptosis in a concentration-dependent manner. Collectively, in HA59T cells, diindolylmethane induced a [Ca] rise by causing phospholipase C-dependent Ca release from the endoplasmic reticulum and Ca influx via phospholipase A-sensitive channels. Diindolylmethane induced cell death that may involve apoptosis.
- Subjects
HEPATOCELLULAR carcinoma; LIVER cells; CALCIUM ions; CELL death; BRASSICACEAE; PREVENTION; THERAPEUTICS
- Publication
Archives of Toxicology, 2011, Vol 85, Issue 10, p1257
- ISSN
0340-5761
- Publication type
Article
- DOI
10.1007/s00204-011-0670-9