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- Title
Characterization of in vitro cultured bone marrow and adipose tissue-derived mesenchymal stem cells and their ability to express neurotrophic factors.
- Authors
Taghi, Ghorbanian Mohammad; Maryam, Ghasem Kashani; Taghi, Lashkarbolouki; Leili, Hosseinpour; Leyla, Mirzaeiyan
- Abstract
MSCs (mesenchymal stem cells) have attracted attention as a promising tool for regenerative medicine and transplantation therapy. MSCs exert neuroprotective effects by secreting a number of factors in vitro and in vivo. Similar characteristics are found in ADSCs (adipose-derived stem cells) and BMSCs (bone marrow stromal cells). Multipotent capability, easy accessibility and rapid proliferation of ADSCs have been established. Our main objective was to compare cell viability, growth rate, expression of neurotrophic factors and nestin genes in ADSCs and BMSCs. Cell doubling time and proliferation rate indicate that ADSCs has a higher proliferation rate than BMSCs. ADSCs and BMSCs express a similar pattern of CD71 and CD90 markers. Nestin immunostaining showed that ADSCs and BMSCs are immunopositive. The expression of neurotrophic factors genes in ADSCs proved similar to that of BMSCs genes. Thus adipose tissue stem cells with a high proliferation rate can express nestin and neurotrophic factor genes. Therefore ADSCs may be useful in future cell replacement therapies and help improve neurodegenerative diseases.
- Subjects
IN vitro studies; BONE marrow; CELL culture; ADIPOSE tissues; MESENCHYMAL stem cells; NEUROTROPHINS; REGENERATIVE medicine; TRANSPLANTATION of organs, tissues, etc.; NEURODEGENERATION
- Publication
Cell Biology International, 2012, Vol 36, Issue 12, p1239
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1042/CBI20110618