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- Title
Genetic analysis of forest species Eugenia uniflora L. through of newly developed SSR markers.
- Authors
Ferreira-Ramos, Ronai; Laborda, Prianda R.; de Oliveira Santos, Melissa; Mayor, Matheus S.; Mestriner, Moacyr A.; de Souza, Anete P.; Alzate-Marin, Ana Lilia
- Abstract
Nine microsatellite loci for genetic analysis of three populations of the tropical tree Eugenia uniflora L. (pitanga or Brazilian cherry) from fragments of semideciduous forest were developed. We used the technique of building a (GA)n and (CA)n microsatellite-enriched library by capture with streptavidin-coated magnetic beads. We assessed the polymorphism of seven microsatellites in 84 mature trees found in three areas (Ribeirã Preto, Tambaú and São José do Rio Pardo), highly impacted by the agricultural practices, in a large region among Pardo river and Mogi-Guaçu river basins, in state of São Paulo, Brazil. All loci were polymorphic, and the number of alleles was high, ranging from 6 to 24, with a mean of 14.4. All stands showed the same high level of genetic diversity (mean HE = 0.83) and a low genetic differentiation (mean FST = 0.031), indicating that genetic diversity was higher within rather than among populations. Seven of the nine loci were highly variable, and sufficiently informative for E. uniflora. It was concluded that these new SSR markers can be efficiently used for gene flow studies.
- Subjects
BRAZIL; GENETIC markers; TREES; MICROSATELLITE repeats; NUCLEOTIDE sequence; GENE libraries
- Publication
Conservation Genetics, 2008, Vol 9, Issue 5, p1281
- ISSN
1566-0621
- Publication type
Article
- DOI
10.1007/s10592-007-9458-0