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- Title
Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome.
- Authors
Lajtai, Krisztina; Nagy, Csilla Terézia; Tarszabó, Róbert; Benkő, Rita; Hadjadj, Leila; Sziva, Réka Eszter; Gerszi, Dóra; Bányai, Bálint; Ferdinandy, Péter; Nádasy, György László; Giricz, Zoltán; Horváth, Eszter Mária; Várbíró, Szabolcs
- Abstract
Aim: We aimed to examine the alterations of the insulin signaling pathway, autophagy, nitrative stress and the effect of vitamin D supplementation in the liver and ovaries of vitamin D deficient hyperandrogenic rats. Methods: Female Wistar rats received eight weeks of transdermal testosterone treatment and lived on a low vitamin D diet (D–T+). Vitamin D supplementation was achieved by oral administration of vitamin D3 (D+T+). Sham-treated (D+T–) and vitamin D deficient animals (D–T–) served as controls. (N = 10–12 per group). Results: D–T+ animals showed decreased LC3 II levels in the liver and increased p-Akt/Akt and p-eNOS/eNOS ratios with decreased insulin receptor staining in the ovaries. Vitamin D supplementation prevented the increase of Akt phosphorylation in the ovaries. Vitamin D deficiency itself also led to decreased LC3 II levels in the liver and decreased insulin receptor staining in the ovaries. D–T+ group showed no increase in nitrotyrosine staining; however, the ovaries of D–T– rats and the liver of D+T+ animals showed increased staining intensity. Conclusion: Vitamin D deficiency itself might lead to disrupted ovarian maturation and autophagy malfunction in the liver. Preventing Akt phosphorylation may contribute to the beneficial effect of vitamin D treatment on ovarian function in hyperandrogenism.
- Subjects
VITAMIN D deficiency; POLYCYSTIC ovary syndrome; CHOLECALCIFEROL; VITAMIN D; INSULIN receptors; OVARIAN follicle
- Publication
Biomolecules (2218-273X), 2019, Vol 9, Issue 9, p471
- ISSN
2218-273X
- Publication type
Article
- DOI
10.3390/biom9090471