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- Title
Variation in the IGF-1 gene is associated with lymphocyte subset counts in neonates: The Generation R Study.
- Authors
Duijts, Liesbeth; Bakker-Jonges, Liesbeth E.; Mook-Kanamori, Dennis O.; Labout, Joost A. M.; Hofman, Albert; van Duijn, Cornelia M.; van Dongen, Jacques J. M.; Hooijkaas, Herbert; Moll, Henriëtte A.; Jaddoe, Vincent W. V.
- Abstract
Objective IGF-1 stimulates growth, development and function of lymphocytes. The aim of this study was to examine whether functional variants of the IGF-1 gene are associated with absolute lymphocyte subset counts in neonates. Study design and measurements This study was embedded in the Generation R Study, a prospective cohort study from foetal life onwards. A polymorphism in the IGF-1 promoter region was genotyped in cord blood DNA. Lymphocytes (T, B and NK) and T lymphocyte subsets (helper, cytotoxic, naïve and memory) in cord blood were immunophenotyped in 380 neonates by six-colour flow cytometry. Results In total, 39% of the neonates were homozygous for the 192-bp allele (wild-type), 48% were heterozygous and 13% were noncarrier. No differences in absolute lymphocyte and T lymphocyte subset counts were observed between the 192-bp allele heterozygous and homozygous groups. In noncarriers, we found 15% lower T lymphocyte ( P = 0·03), 22% lower B lymphocyte ( P = 0·04) and 10% lower NK lymphocyte counts ( P = 0·36) than in the 192-bp allele homozygous group. Analyses of T lymphocyte subsets showed 16% lower helper T lymphocyte counts ( P = 0·01) in noncarriers. No significant differences were found for cytotoxic, naïve and memory T lymphocyte counts. All associations were adjusted for gravidity, mode of delivery, gestational age, birth weight, gender and 1- and 5- min Apgar scores. Conclusions Our study showed associations between this IGF-1 promoter region polymorphism and absolute lymphocyte subset counts in neonates. These results should be regarded as hypothesis generating until they have been replicated in other studies.
- Subjects
LYMPHOCYTES; GENES; GENETIC polymorphisms; NEWBORN infants; T cells
- Publication
Clinical Endocrinology, 2009, Vol 70, Issue 1, p53
- ISSN
0300-0664
- Publication type
Article
- DOI
10.1111/j.1365-2265.2008.03294.x