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- Title
Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.
- Authors
Lu, Quanlong; Insinna, Christine; Ott, Carolyn; Stauffer, Jimmy; Pintado, Petra A.; Rahajeng, Juliati; Baxa, Ulrich; Walia, Vijay; Cuenca, Adrian; Hwang, Yoo-Seok; Daar, Ira O.; Lopes, Susana; Lippincott-Schwartz, Jennifer; Jackson, Peter K.; Caplan, Steve; Westlake, Christopher J.
- Abstract
Membrane association with mother centriole (M-centriole) distal appendages is critical for ciliogenesis initiation. How the Rab GTPase Rab11-Rab8 cascade functions in early ciliary membrane assembly is unknown. Here, we show that the membrane shaping proteins EHD1 and EHD3, in association with the Rab11-Rab8 cascade, function in early ciliogenesis. EHD1 and EHD3 localize to preciliary membranes and the ciliary pocket. EHD-dependent membrane tubulation is essential for ciliary vesicle formation from smaller distal appendage vesicles (DAVs). Importantly, this step functions in M-centriole to basal body transformation and recruitment of transition zone proteins and IFT20. SNAP29, a SNARE membrane fusion regulator and EHD1-binding protein, is also required for DAV-mediated ciliary vesicle assembly. Interestingly, only after ciliary vesicle assembly is Rab8 activated for ciliary growth. Our studies uncover molecular mechanisms informing a previously uncharacterized ciliogenesis step, whereby EHD1 and EHD3 reorganize the M-centriole and associated DAVs before coordinated ciliary membrane and axoneme growth.
- Subjects
CILIARY body; VESICLE associated membrane protein; AXONEMES; CARRIER proteins; MOLECULAR structure
- Publication
Nature Cell Biology, 2015, Vol 17, Issue 3, p228
- ISSN
1465-7392
- Publication type
Article
- DOI
10.1038/ncb3109