We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
mRNA-electroporated mature dendritic cells retain transgene expression, phenotypical properties and stimulatory capacity after cryopreservation.
- Authors
Ponsaerts, P.; Van Tendeloo, V.F.I.; Cools, N.; Van Dierssche, A.; Lardon, F.; Nijs, G.; Lenjou, M.; Mertens, G.; Van Broeckhoven, C.; Van Bockstaele, D.R.; Berneman, Z.N.
- Abstract
Genetically modified dendritic cells (DC) are increasingly used in vitro to activate cytotoxic T lymphocyte (CTL) immune responses. Because T cell activation protocols consist of multiple restimulation cycles of peripheral blood lymphocytes with antigen-loeded mature DC, continuous generation of DC is needed throughout the experiment. Therefore, cryopreservation of DC loaded with antigen is a valuable alternative for weekly generation and modification of DC. Recently, we described an antigen loading method for DC based on electroporation of defined tumor antigen mRNA. In this study, we demonstrate that mRNA-electroporated DC can efficiently be prepared for cryopreservation. Using an optimized maturation and freezing protocol after mRNA electroporation, we obtained high transgene-expreesing viable mature DC. In addition, we showed that these modified cryopreserved DC retain stimulatory capacity in an influenza model system. Therefore, cryopreservation of mature mRNA-electroporated DC is a useful method for continuous availability of antigen-loaded DC throughout T cell activation experiments.
- Subjects
DENDRITIC cells; CRYOPRESERVATION of organs, tissues, etc.; MESSENGER RNA
- Publication
Leukemia (08876924), 2002, Vol 16, Issue 7, p1324
- ISSN
0887-6924
- Publication type
Article
- DOI
10.1038/sj.leu.2402511