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- Title
dCas9 techniques for transcriptional repression in mammalian cells: Progress, applications and challenges.
- Authors
Li, Yuanyuan; Zhou, Li‐quan
- Abstract
Innovative loss‐of‐function techniques developed in recent years have made it much easier to target specific genomic loci at transcriptional levels. CRISPR interference (CRISPRi) has been proven to be the most effective and specific tool to knock down any gene of interest in mammalian cells. The catalytically deactivated Cas9 (dCas9) can be fused with transcription repressors to downregulate gene expression specified by sgRNA complementary to target genomic sequence. Although CRISPRi has huge potential for gene knockdown, there is still a lack of systematic guidelines for efficient and widespread use. Here we describe the working mechanism and development of CRISPRi, designing principles of sgRNA, delivery methods and applications in mammalian cells in detail. Finally, we propose possible solutions and future directions with regard to current challenges.
- Subjects
CRISPRS; GENE expression; GENOME editing
- Publication
BioEssays, 2021, Vol 43, Issue 9, p1
- ISSN
0265-9247
- Publication type
Article
- DOI
10.1002/bies.202100086