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- Title
Bioprinting of three-dimensional dentin–pulp complex with local differentiation of human dental pulp stem cells.
- Authors
Han, Jonghyeuk; Kim, Da Sol; Jang, Ho; Kim, Hyung-Ryong; Kang, Hyun-Wook
- Abstract
Numerous approaches have been introduced to regenerate artificial dental tissues. However, conventional approaches are limited when producing a construct with three-dimensional patient-specific shapes and compositions of heterogeneous dental tissue. In this research, bioprinting technology was applied to produce a three-dimensional dentin–pulp complex with patient-specific shapes by inducing localized differentiation of human dental pulp stem cells within a single structure. A fibrin-based bio-ink was designed for bioprinting with the human dental pulp stem cells. The effects of fibrinogen concentration within the bio-ink were investigated in terms of printability, human dental pulp stem cell compatibility, and differentiation. The results show that micro-patterns with human dental pulp stem cells could be achieved with more than 88% viability. Its odontogenic differentiation was also regulated according to the fibrinogen concentration. Based on these results, a dentin–pulp complex having patient-specific shape was produced by co-printing the human dental pulp stem cell–laden bio-inks with polycaprolactone, which is a bio-thermoplastic used for producing the overall shape. After culturing with differentiation medium for 15 days, localized differentiation of human dental pulp stem cells in the outer region of the three-dimensional cellular construct was successfully achieved with localized mineralization. This result demonstrates the possibility to produce patient-specific composite tissues for tooth tissue engineering using three-dimensional bioprinting technology.
- Subjects
DENTAL pulp; BIOPRINTING; STEM cells; DENTIN; TISSUE engineering; TEETH
- Publication
Journal of Tissue Engineering, 2019, Vol 10, pN.PAG
- ISSN
2041-7314
- Publication type
Article
- DOI
10.1177/2041731419845849