We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Association of IL-4 and IL-10 maternal haplotypes with immune responses to P. falciparum in mothers and newborns.
- Authors
Gatien Lokossou, Adjimon; Dechavanne, Célia; Bouraïma, Aziz; Courtin, David; Le Port, Agnès; Ladékpo, Rodolphe; Noukpo, Julien; Bonou, Désiré; Ahouangninou, Claude; Sabbagh, Audrey; Fayomi, Benjamin; Massougbodji, Achille; Garcia, André; Migot-Nabias, Florence
- Abstract
Background: Particular cytokine gene polymorphisms are involved in the regulation of the antibody production. The consequences of already described IL-4, IL-10 and IL-13 gene polymorphisms on biological parameters and antibody levels were investigated among 576 mothers at delivery and their newborns in the context of P. falciparum placental malaria infection. Methods: The study took place in the semi-rural area of Tori-Bossito, in south-west Benin, where malaria is meso-endemic. Six biallelic polymorphisms were determined by quantitative PCR using TaqManW Pre-Designed SNP Genotyping Assays, in IL-4 (rs2243250, rs2070874), IL-10 (rs1800896, rs1800871, rs1800872) and IL-13 (rs1800925) genes. Antibody responses directed to P. falciparum MSP-1, MSP-2, MSP-3, GLURP-R0, GLURP-R2 and AMA-1 recombinant proteins were determined by ELISA. Results: The maternal IL-4-590*T/IL-4+33*T haplotype (one or two copies) was associated with favorable maternal condition at delivery (high haemoglobin levels, absence of placental parasites) and one of its component, the IL-4-590 TT genotype, was related to low IgG levels to MSP-1, MSP-2/3D7 and MSP-2/FC27. Inversely, the maternal IL-10-1082 AA was positively associated with P. falciparum placenta infection at delivery. As a consequence, the IL-10-819*T allele (in CT and TT genotypes) as well as the IL-10-1082*A/IL-10-819*T/IL-10-592*A haplotype (one or two copies) in which it is included, were related to an increased risk for anaemia in newborns. The maternal IL-10-1082 AA genotype was related to high IgG levels to MSP-2/3D7 and AMA-1 in mothers and newborns, respectively. The IL-13 gene polymorphism was only involved in the newborn's antibody response to AMA-1. Conclusion: These data revealed that IL-4 and IL-10 maternal gene polymorphisms are likely to play a role in the regulation of biological parameters in pregnant women at delivery (anaemia, P. falciparum placenta infection) and in newborns (anaemia). Moreover, IL-4, IL-10 and IL-13 maternal gene polymorphisms were related to IgG responses to MSP-1, MSP-2/3D7 and MSP-2/FC27 in mothers as well as to AMA-1 in newborns.
- Subjects
BENIN; CYTOKINES; GENETIC polymorphisms; IMMUNOGLOBULINS; MALARIA prevention
- Publication
BMC Infectious Diseases, 2013, Vol 13, Issue 1, p1
- ISSN
1471-2334
- Publication type
Article
- DOI
10.1186/1471-2334-13-215