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- Title
Single-cell zeroth-order protein degradation enhances the robustness of synthetic oscillator.
- Authors
Wong, Wilson W; Tsai, Tony Y; Liao, James C
- Abstract
In Escherichia coli, protein degradation in synthetic circuits is commonly achieved by the ssrA-tagged degradation system. In this work, we show that the degradation kinetics for the green fluorescent protein fused with the native ssrA tag in each cell exhibits the zeroth-order limit of the Michaelis–Menten kinetics, rather than the commonly assumed first-order. When measured in a population, the wide distribution of protein levels in the cells distorts the true kinetics and results in a first-order protein degradation kinetics as a population average. Using the synthetic gene-metabolic oscillator constructed previously, we demonstrated theoretically that the zeroth-order kinetics significantly enlarges the parameter space for oscillation and thus enhances the robustness of the design under parametric uncertainty.
- Subjects
GREEN fluorescent protein; ROBUST control; ESCHERICHIA coli; DYNAMICS; CELLS
- Publication
Molecular Systems Biology, 2007, Vol 3, Issue 1, p130
- ISSN
1744-4292
- Publication type
Article
- DOI
10.1038/msb4100172