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- Title
Leukocytapheresis Therapy Modulates Circulating T cell Subsets in Patients With Ulcerative Colitis.
- Authors
Andoh, Akira; Tsujikawa, Tomoyuki; Inatomi, Osamu; Deguchi, Yasuyuki; Sasaki, Masaya; Obata, Hirozumi; Mitsuyama, Keiichi; Fujiyama, Yoshihide
- Abstract
The aim of this study was to elucidate the molecular mechanisms responsible for the therapeutic effects of leukocytapheresis (LCAP). We investigated the alterations in circulating T cell subsets after LCAP therapy in ulcerative colitis (UC) patients. Eighteen patients with UC were enrolled. Fourteen patients were responders, and four patients were non-responders. Peripheral venous blood was obtained within 5 min before and 5 min after LCAP therapy. Flow cytometric analysis for T cell markers and intracellular interferon (IFN)-γ (Th1) and interleukin (IL)-4 (Th2) was then performed. The average numbers of lymphocytes, T and B cells were significantly decreased after LCAP therapy, respectively ( P < 0.01). The numbers of CD4+ and CD8+ T cells were also significantly decreased, respectively ( P < 0.01), but the CD4+/CD8+ ratio was not changed. The number of CD45RO+CD4+ memory T cells was significantly decreased. The number of CD25+CD4+ T cells tended to decrease after LCAP therapy (not significant). However, the ratio of CD25+CD4+-cells/CD25–CD4+-cells was significantly increased ( P < 0.05). The number of IFN-γ-positive (Th1) cells was significantly decreased after LCAP therapy, but there was no significant change in the number of IL-4-positive (Th2) cells. The Th1/Th2 ratio was significantly decreased after LCAP therapy. Some of the immuno-suppressive effects of LCAP therapy may be associated with a modulation of circulating T cell subsets.
- Subjects
THERAPEUTICS; T cells; ANTIVIRAL agents; LYMPHOCYTES; INFLAMMATORY bowel diseases; ANTINEOPLASTIC agents
- Publication
Therapeutic Apheresis & Dialysis, 2005, Vol 9, Issue 3, p270
- ISSN
1744-9979
- Publication type
Article
- DOI
10.1111/j.1774-9987.2005.00270.x