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- Title
The Lipid A from Rhodopseudomonas palustris Strain BisA53 LPS Possesses a Unique Structure and Low Immunostimulant Properties.
- Authors
Di Lorenzo, Flaviana; Palmigiano, Angelo; Al Bitar-Nehme, Sami; Sturiale, Luisa; Duda, Katarzyna A.; Gully, Djamel; Lanzetta, Rosa; Giraud, Eric; Garozzo, Domenico; Bernardini, Maria Lina; Molinaro, Antonio; Silipo, Alba
- Abstract
The search for novel lipid A analogues from any biological source that can act as antagonists, displaying inhibitory activity towards the production of pro-inflammatory cytokines, or as immunomodulators in mammals, is a very topical issue. To this aim, the structure and immunological properties of the lipopolysaccharide lipid A from the purple nonsulfur bacterium Rhodopseudomonas palustris strain BisA53 have been determined. This lipid A displays a unique structural feature, with a non-phosphorylated skeleton made up of the tetrasaccharide Man p-α-(1→4)-Glc pN3N-β-1→6-Glc pN3N-α-(1→1)-α-Gal pA, and four primary amide-linked 14:0(3-OH) and, as secondary O-acyl substituents, a 16:0 and the very long-chain fatty acid 26:0(25-OAc), appended on the Glc pN3N units. This lipid A architecture is definitely rare, so far identified only in the genus Bradyrhizobium. Immunological tests on both murine bone-marrow-derived and human monocyte-derived macrophages revealed an extremely low immunostimulant capability of this LPS lipid A.
- Subjects
RHODOPSEUDOMONAS palustris; LIPID synthesis; INNATE lymphoid cells; IMMUNOREGULATION; CONNECTIVE tissue cells; HEMATOPOIETIC system
- Publication
Chemistry - A European Journal, 2017, Vol 23, Issue 15, p3637
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.201604379