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- Title
Detection of nano-second internal motion and determination of overall tumbling times independent of the time scale of internal motion in proteins from NMR relaxation data.
- Authors
Larsson, Göran; Martinez, Gary; Schleucher, Jürgen; Wijmenga, Sybren S.
- Abstract
The usual analysis of 15N relaxation data of proteins is straightforward as long as the assumption can be made that the backbone of most residues only undergoes fast (ps), small amplitude internal motions. If this assumption cannot be made, as for example for proteins which undergo domain motions or for unfolded or partially folded proteins, one needs a method to establish for each residue whether it undergoes fast (ps) or slow (ns) internal motion. Even then it is impossible to determine the correct overall tumbling time, τm0, via the usual method from the ratio of the longitudinal and transverse relaxation times, if the majority of residues do not undergo fast, small amplitude internal motions. The latter problem is solved when τm0 can be determined independent of the time scale, τi, or the amplitude, S2, of the internal motion. We propose a new protocol, called PINATA, for analyzing 15N relaxation data acquired at minimally two field strengths, where no a priori assumption about time scales or amplitude of internal motions needs to be made, and overall tumbling can either be isotropic or anisotropic. The protocol involves four steps. First, for each residue, it is detected whether it undergoes ps- or ns-internal motion, via the combination of the ratio of the longitudinal relaxation time at two fields and the hetero-nuclear NOE. Second, for each residue τm0 and the exchange broadening, Rex, are iteratively determined. The accuracy of the determination of τm0 is ca. ±0.5 ns and of Rex ca ± 0.7 s-1, when the relaxation data are of good quality and τm0>5 ns, S2>0.3, and τi<≈3 ns. Third, given τm0 and Rex, step 1 is repeated to iteratively improve on the internal motion and obtain better estimates of the internal parameter values. Fourth, final time scales and amplitudes for internal motions are determined via grid search based fitting and χ2-analysis. The protocol was successfully tested on synthetic and experimental data sets. The synthetic dataset mimics internal motions on either fast or slow time scales, or a combination of both, of either small- or large amplitude, superimposed onto anisotropic overall motion. The procedures are incorporated into MATLAB scripts, which are available on request.
- Subjects
PROTEINS; NUCLEAR magnetic resonance; RELAXATION spectroscopy; BIOMOLECULES; SPECTRUM analysis; QUANTUM theory
- Publication
Journal of Biomolecular NMR, 2003, Vol 27, Issue 4, p291
- ISSN
0925-2738
- Publication type
Article
- DOI
10.1023/A:1025836018993