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- Title
Evaluation of Anticancer Activity of Nucleoside–Nitric Oxide Photo-Donor Hybrids.
- Authors
Marchesi, Elena; Melloni, Elisabetta; Casciano, Fabio; Pozza, Elena; Argazzi, Roberto; De Risi, Carmela; Preti, Lorenzo; Perrone, Daniela; Navacchia, Maria Luisa
- Abstract
Herein, we report the synthesis of a new hybrid compound based on a 2′-deoxyuridine nucleoside conjugated with a NO photo-donor moiety (dU-t-NO) via CuAAC click chemistry. Hybrid dU-t-NO, as well as two previously reported 2′-deoxyadenosine based hybrids (dAdo-S-NO and dAdo-t-NO), were evaluated for their cytotoxic and cytostatic activities in selected cancer cell lines. dAdo-S-NO and dAdo-t-NO hybrids displayed higher activity with respect to dU-t-NO. All hybrids showed effective release of NO in the micromolar range. The photochemical behavior of the newly reported hybrid, dU-t-NO, was studied in the RKO colon carcinoma cell line, whereas the dAdo-t-NO hybrid was tested in both colon carcinoma RKO and hepatocarcinoma Hep 3B2.1-7 cell lines to evaluate the potential effect of NO released upon irradiation on cell viability. A customized irradiation apparatus for in vitro experiments was also designed.
- Subjects
CLICK chemistry; CELL lines; ANTINEOPLASTIC agents; CELL survival; NITRIC oxide
- Publication
Molecules, 2024, Vol 29, Issue 14, p3383
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules29143383