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- Title
mRNA encoding WAVE-Arp2/3-associated proteins is co-localized with foci of active protein synthesis at the leading edge of MRC5 fibroblasts during cell migration.
- Authors
WILLETT, Mark; BROCARD, Michele; POLLARD, Hilary J.; MORLEY, Simon J.
- Abstract
During cell spreading, mammalian cells migrate using lamellipodia formed from a large dense branched actin network which produces the protrusive force required for leading edge advancement. The formation of lamellipodia is a dynamic process and is dependent on a variety of protein cofactors thatmediate their local regulation, structural characteristics and dynamics. In the present study, we show thatmRNAs encoding some structural and regulatory components of the WAVE [WASP (Wiskott-Aldrich syndrome protein) verprolin homologous] complex are localized to the leading edge of the cell and associated with sites of active translation. Furthermore, we demonstrate that steady-state levels of ArpC2 and Rac1 proteins increase at the leading edge during cell spreading, suggesting that localized protein synthesis has a pivotal role in controlling cell spreading and migration.
- Subjects
LAMELLIPODIA; COFACTORS (Biochemistry); WISKOTT-Aldrich syndrome protein; GENETIC translation; PROTEIN synthesis; RAS-related nuclear protein
- Publication
Biochemical Journal, 2013, Vol 452, Issue 1, p45
- ISSN
0264-6021
- Publication type
Article
- DOI
10.1042/BJ20121803