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- Title
RNA Oligomerisation without Added Catalyst from 2′,3′‐Cyclic Nucleotides by Drying at Air‐Water Interfaces.
- Authors
Dass, Avinash Vicholous; Wunnava, Sreekar; Langlais, Juliette; von der Esch, Beatriz; Krusche, Maik; Ufer, Lennard; Chrisam, Nico; Dubini, Romeo C. A.; Gartner, Florian; Angerpointner, Severin; Dirscherl, Christina F.; Rovó, Petra; Mast, Christof B.; Šponer, Judit E.; Ochsenfeld, Christian; Frey, Erwin; Braun, Dieter
- Abstract
For the emergence of life, the abiotic synthesis of RNA from its monomers is a central step. We found that in alkaline, drying conditions in bulk and at heated air‐water interfaces, 2′,3′‐cyclic nucleotides oligomerised without additional catalyst, forming up to 10‐mers within a day. The oligomerisation proceeded at a pH range of 7–12, at temperatures between 40–80 °C and was marginally enhanced by K+ ions. Among the canonical ribonucleotides, cGMP oligomerised most efficiently. Quantification was performed using HPLC coupled to ESI‐TOF by fitting the isotope distribution to the mass spectra. Our study suggests a oligomerisation mechanism where cGMP aids the incorporation of the relatively unreactive nucleotides C, A and U. The 2′,3′‐cyclic ribonucleotides are byproducts of prebiotic phosphorylation, nucleotide syntheses and RNA hydrolysis, indicating direct recycling pathways. The simple reaction condition offers a plausible entry point for RNA to the evolution of life on early Earth.
- Subjects
RNA analysis; OLIGOMERIZATION; AIR-water interfaces; CYCLIC nucleotides; DRYING; PREBIOTICS
- Publication
ChemSystemsChem, 2023, Vol 5, Issue 1, p1
- ISSN
2570-4206
- Publication type
Article
- DOI
10.1002/syst.202200026