We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Improving the secretion of cadaverine in Corynebacterium glutamicum by cadaverine-lysine antiporter.
- Authors
Li, Ming; Li, Dongxia; Huang, Yunyan; Liu, Meng; Wang, Hongxin; Tang, Qi; Lu, Fuping
- Abstract
Cadaverine (1,5-pentanediamine, diaminopentane), the desired raw material of bio-polyamides, is an important industrial chemical with a wide range of applications. Biosynthesis of cadaverine in Corynebacterium glutamicum has been a competitive way in place of petroleum-based chemical synthesis method. To date, the cadaverine exporter has not been found in C. glutamicum. In order to improve cadaverine secretion, the cadaverine-lysine antiporter CadB from Escherichia coli was studied in C. glutamicum. Fusion expression of cadB and green fluorescent protein (GFP) gene confirmed that CadB could express in the cell membrane of C. glutamicum. Co-expression of cadB and ldc from Hafnia alvei in C. glutamicum showed that the cadaverine secretion rate increased by 22 % and the yield of total cadaverine and extracellular cadaverine increased by 30 and 73 %, respectively. Moreover, the recombinant strain cultured at acid and neutral pH separately hardly had any difference in cadaverine concentrations. These results suggested that CadB could be expressed in the cell membrane of C. glutamicum and that recombinant CadB could improve cadaverine secretion and the yield of cadaverine. Moreover, the pH value did not affect the function of recombinant CadB. These results may be a promising metabolic engineering strategy for improving the yield of the desired product by enhancing its export out of the cell.
- Subjects
CORYNEBACTERIUM glutamicum; LYSINE; POLYAMIDES; CHEMICAL synthesis; PETROLEUM; GREEN fluorescent protein; BACTERIAL cell membranes
- Publication
Journal of Industrial Microbiology & Biotechnology, 2014, Vol 41, Issue 4, p701
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1007/s10295-014-1409-4