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- Title
Investigation of the role of Toxoplasma gondii infection in the pathophysiology of Alzheimer's disease.
- Authors
Çetinkaya, Ayhan; Gökdaş, Serhat; Öğün, Muhammed Nur; Yaman, Kerem; Türkoğlu, Şule Aydın; Türel, Canan Akünal; Demir, Şerif; Ayaz, Erol
- Abstract
Objective: Alzheimer's disease (AD) is a neurodegenerative disease that manifests itself with cognitive symptoms by affecting the cerebral cortex and hippocampus environment. amyloid plaques, neurofibrillary tangles and neuronal cell death play a role in the pathophysiological mechanism of AD. T. gondii is an important zoonotic parasite that settles in cardiac muscle, smooth muscle, eye and brain. The aim of the study is to investigate the behavioral and histopathological effects of pre-pregnancy T. gondii infection in the adult periods of the offspring to be born during and after pregnancy. Similar to the study, the effects of the effects of the parasite taken during pregnancy on mothers were investigated and the results were brought to the literature. The originality of our study was based on the offspring of the parasite taken before pregnancy, during and after pregnancy, and targeted the gap in the literature. Methods: There are 5 groups in total: Group 1 (Mother Control), Group 2 (Mother Alzheimer's), Group 3 (Mother Toxoplasma), Group 4 (Mother Alzhimer+Toxoplasma), Group 5 (Mother Toxoplasma+Alzhimer). For Alzheimer's model, animals were given Aβ1-42 solution 4 μg/1 μL/side bilaterally intrahippocampally, and for T. gondii infection, an inoculum containing 1.5¥106 parasites was prepared and administered intraperitoneally. Mother Groups were mated with healthy male rats simultaneously, and our experiment was planned as 8 puppies each according to power analysis. Our study tested whether the animals infected with T. gondii trigger Alzheimer's-like behaviors by detecting the presence of beta amyloid clusters in the brain according to the histopathological staining method with learning and memory tests when the pups reach adulthood. Results: Although there was no statistical difference between Group1 (pup) and Group 4 (pup), according to the behavioral test (p>0.05). The difference between Group 1 and other groups was determined statistically (p<0.05). Although Aβ cluster was determined except for Group 1 according to Congo Red histological staining method, there was no statistically significant difference between the offspring groups (p>0.05). Conclusion: In the light of these data, T. gondii parasite showed Alzheimer's-like morphological changes, but it was not statistically significant.
- Subjects
ALZHEIMER'S disease; NEUROFIBRILLARY tangles; TOXOPLASMA gondii; PATHOLOGICAL physiology; STAINS &; staining (Microscopy); AMYLOID plaque
- Publication
Anatomy: International Journal of Experimental & Clinical Anatomy, 2022, Vol 16, p282
- ISSN
1307-8798
- Publication type
Article
- DOI
10.2399/ana.22.003s