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- Title
Temperature-responsive amperometric HO biosensor using a composite film consisting of poly(N-isopropylacrylamide)-b-poly (2-acrylamidoethyl benzoate), graphene oxide and hemoglobin.
- Authors
Zhou, Yuanqing; Cao, Jing; Zhao, Jia; Xie, Yixi; Fei, Junjie; Cai, Yuanli
- Abstract
A temperature-responsive biosensing film consisting of the temperature-responsive block co-polymer poly (N-isopropylacrylamide)-b-poly(2-acrylamidoethyl benzoate) (referred to as PNIPAM-b-PAAE), graphene oxide (GO), and hemoglobin (Hb) was fabricated and used to modify a glassy carbon electrode (GCE). The film provides a favorable micro-environment for Hb to facilitate the electron transfer to the GCE. Hb at PNIPAM-b-PAAE/GO/Hb (PGH) film exhibits a couple of well-defined redox peaks with a formal potential of −0.371 V (vs. SCE) and displays intrinsic electro-catalytic activity toward HO. The sensing film also shows temperature-tunable catalytic activity toward HO that can be stimulated by temperature. Large peak currents can be seen in amperometry at 0.4 V (vs. SCE) in pH 7.0 phosphate buffer only if the temperature is above the lower critical solution temperature (LCST) of 32 °C. The response of the modified GCE is linear in the 0.1 to 3.7 μmol L concentration range if operated at above 32 °C, but in the 0.2 to 3.7 μmol L concentration range at below 30 °C. This behavior is attributed to the temperature-dependent phase transition of PNIPAM-b-PAAE and cooperative effect of GO. The strategy presented here in our perception meets the requirements of switchable sensors for use in bioscience and biotechnology.
- Subjects
THERMORESPONSIVE polymers; CONDUCTOMETRIC analysis; BIOSENSORS; BENZOATES; CATALYTIC activity
- Publication
Microchimica Acta, 2016, Vol 183, Issue 9, p2501
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-016-1893-5