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- Title
Performance Evaluation and Clinical Associations of Immunoassays That Detect Antibodies to Negatively Charged Phospholipids Other Than Cardiolipin.
- Authors
Castanon, Amaris; Pierre, Grant; Willis, Rohan; Harris, E Nigel; Papalardo, Elizabeth; Romay-Penabad, Zurina; Schleh, Alvaro; Jajoria, Praveen; Smikle, Monica; DeCeulaer, Karel; Tebo, Anne; Jaskowski, Troy; Guerra, Marta M; Branch, D Ware; Salmon, Jane E; Petri, Michelle; Gonzalez, Emilio B
- Abstract
<bold>Objectives: </bold>We evaluate the performance characteristics of antiphosphatidylserine (anti-PS), antiphosphatidylinositol (anti-PI), and antiphospholipid mixture (APhL) enzyme-linked immunosorbent assays (ELISAs) compared with anticardiolipin (aCL) and anti-β2 glycoprotein I (anti-β2GPI) in a large group of patients with antiphospholipid (aPL)-related diseases.<bold>Methods: </bold>Serum samples from 548 patients from the Hopkins and Jamaican systemic lupus erythematosus cohorts, the PROMISSE cohort, and the Antiphospholipid Standardization Laboratory were examined for immunoglobulin G (IgG)/immunoglobulin M (IgM) positivity in aCL, anti-β2GPI, anti-PS, anti-PI, and APhL ELISA assays.<bold>Results: </bold>All IgG assays were associated with one or more thrombotic and/or obstetric manifestations, with an increased risk associated with higher antibody titers. Analytical performance was similar among assays, but IgG assays performed better than IgM counterparts.<bold>Conclusions: </bold>Increasing titers of APhL, anti-PS, and anti-PI antibodies could indicate an increased risk of thrombotic and/or obstetric aPL-related manifestations. These assays may be promising biomarkers for particular APS manifestations.
- Subjects
IMMUNOASSAY; PHOSPHOLIPIDS; CARDIOLIPIN; AUTOANTIBODIES; COMPARATIVE studies; ENZYME-linked immunosorbent assay; GLYCOPROTEINS; IMMUNOGLOBULINS; LONGITUDINAL method; RESEARCH methodology; MEDICAL cooperation; RESEARCH; RESEARCH funding; SYSTEMIC lupus erythematosus; THROMBOSIS; EVALUATION research
- Publication
American Journal of Clinical Pathology, 2018, Vol 149, Issue 5, p401
- ISSN
0002-9173
- Publication type
journal article
- DOI
10.1093/ajcp/aqy003