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- Title
Proteomic analysis and characterisation of human cervico-vaginal fluid proteins.
- Authors
di Quinzio, Megan K. W.; Oliva, Karen; Holdsworth, Sarah J.; Ayhan, Mustafa; Walker, Susan P.; Rice, Gregory E.; Georgiou, Harry M.; Permezel, Michael
- Abstract
Aim: Cervico-vaginal fluid (CVF) may provide insight into the biochemical pathways of human reproduction and parturition. The aim of this study was to establish a 2-D electrophoretic map of human CVF in healthy, pregnant women at term. Methods: CVF was collected, concentrated and processed by routine 2-D polyacrylamide gel electrophoresis using pH 4–7-immobilised pH gradient strips and 8–16% gradient polyacrylamide gels. Imaged gels were analysed, yielding more than 400 proteins. A total of 157 proteins were common to all gels with a subgroup of the most abundant proteins being excised and characterised either by MALDI or by electrospray ion-trap mass spectrometry. Results: Twenty-one proteins were successfully identified, yielding 15 different proteins. These included blood transport proteins (albumin and transthyretin); a structural protein (β-actin); proteins involved in fatty acid metabolism (fatty acid-binding protein and acetyl-CoA-binding protein); a calcium-binding protein (annexin III); an anti-inflammatory cytokine (interleukin-1 receptor antagonist); proteinase inhibitors (α-1-antitrypsin, monocyte/neutrophil elastase inhibitor, squamous cell carcinoma antigen-1 and cystatin A); and enzymes involved in oxidative stress defence (thioredoxin, peroxiredoxin 2, glutathione S-transferase P and copper,zinc superoxide dismutase). Conclusion: CVF is a complex body fluid consisting of both endogenous and environmental proteins. The putative role of some of these proteins in the human reproductive tract is discussed.
- Subjects
FEMALE reproductive organ secretions; BODY fluids; PROTEOMICS; TWO-dimensional electrophoresis; PREGNANT women; PENNING trap mass spectrometry; PARTURITION; HUMAN reproduction
- Publication
Australian & New Zealand Journal of Obstetrics & Gynaecology, 2007, Vol 47, Issue 1, p9
- ISSN
0004-8666
- Publication type
Article
- DOI
10.1111/j.1479-828X.2006.00671.x