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- Title
Human placenta: de visudemonstration of interstitial Cajal-like cells.
- Authors
Suciu, Laura; Popescu, L. M.; Gherghiceanu, Mihaela
- Abstract
Traditional interstitial cells of Cajal (ICC) are present in the digestive tube and are supposed to act as pacemakers and neuromodulators. However, interstitial Cajal-like cells (ICLCs) were found outside the gastrointestinal tract, in various organs ( e.g. ureter, bladder, fallopian tube, uterus, pancreas, mammary gland, myocardium etc.) and looking for such ICLC is a priority in our laboratories. We report here unequivocal visual evidence that ICLCs are present in the mesenchymal tissue of the villi from human term placenta. The following methods were used: a. vital staining with methylene blue (cryosections); b. silver impregnation(paraffin sections); c. Epon-embedded sections (∼1μm) of glutaraldehyde/osmium fixed tissue, stained with toluidine blue; d. primary cell cultures (or second-passage cells) to reveal the characteristic, very long, moniliform cell processes and mitochondrial localization at dilations (molecular fluorescence probe: MitoTracker Green); e. immunofluorescence for c-kit/CD117 marker or other characteristic proteins; f. transmission electron microscopy to establish the identity of ICLC.
- Subjects
PLACENTA; CELLS; PACEMAKER cells; GASTROINTESTINAL system; URETERS; FALLOPIAN tubes; UTERUS; MAMMARY glands
- Publication
Journal of Cellular & Molecular Medicine, 2007, Vol 11, Issue 3, p590
- ISSN
1582-1838
- Publication type
Article
- DOI
10.1111/j.1582-4934.2007.00058.x