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- Title
Freezing artifact in Muscle Biopsy Specimens - a Novel Approach.
- Authors
Sandberg, Glenn D.; Rushing, Elisabeth J.; Horkayne-Szakaly, Iren
- Abstract
Objectives: Muscle biopsy is an important diagnostic tool in neuromuscular diseases. However, frozen specimens are subject to artifact that may hinder optimal interpretation. Our goal is to evaluate whether Aedesta - Cell/Tissue Preservation solution (ACTP) reduces artifacts by restoring normal myofiber structure to muscle biopsy specimens and preserving enzymatic activity and immunoreactivity. Materials and methods: We studied five human muscle biopsy specimens, which showed marked freezing artifact limiting evaluation. The specimens were gradually defrosted from -70 to 4 °C, treated with ACTP. After treatment, each biopsy specimen was evaluated with a standard panel of 8 enzyme histochemical stains and the MHC1 immunohistochemical preparation. Results: Biopsy specimens treated with ACTP solution exhibited fewer ice crystal artifacts. Myofiber structure, enzymatic activity and immunoreactivity were restored compared to their previous state. Conclusion: This study demonstrates that ACTP solution can reverse slow-freeze artifact and could be beneficial in routine processing of skeletal muscle biopsies. Accordingly, further evaluation of this agent is indicated.
- Publication
FASEB Journal, 2008, Vol 22, p586
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fasebj.22.2_supplement.586